Keywords
cladodes
phytochemicals
pharmacological activities
bioactive compounds
How to Cite
Abstract
IntroductionOpuntia ficus-indica cladodes are widely used in food systems and traditional medicine across Africa, Latin America, and the Mediterranean. Their high content of bioactive compounds has attracted increasing scientific attention due to reported pharmacological properties, including antioxidant, anti-inflammatory, antimicrobial, and antidiabetic activities. However, current evidence remains fragmented and heterogeneous, limiting clear understanding of the relationships between phytochemicals and their mechanisms of action.
Purpose
This study provides a critical and integrative review of the phytochemical composition, pharmacological activities, and mechanisms of action of O. ficus-indica cladodes.
Methods
A structured literature review was conducted using ScienceDirect, PubMed, Google Scholar, and WHO reports. Keywords included “Opuntia ficus-indica cladodes,” “phytochemical composition,” “bioactive compounds,” and “pharmacological activity.” Peer-reviewed articles and reports published between 2014 and 2025 were included, while studies focusing on non-cladode plant parts were excluded. References were managed using Zotero.
Results
Cladodes contain diverse phenolic compounds, including p-coumaric acid (14.08–16.18 mg/100 g), narcissin (14.69–137.10 mg/100 g), rutin (2.36–26.17 mg/100 g), isoquercetin (2.29–39.67 mg/100 g), isorhamnetin derivatives, and nicotiflorin (2.89–146.50 mg/100 g), which significantly contribute to their bioactivity. Major phytosterols identified include β-sitosterol (16.17 mg/kg), stigmasterol (16.53 mg/kg), Δ7-avenasterol (13.40 mg/kg), and campesterol (11.60 mg/kg). Carbohydrates such as glucose (153.15 ± 6.50 µg/mg), xylose, and mannose, along with minerals including calcium (5.64–316.95 mg/100 g), iron, zinc, and manganese, further enhance their functional potential. Antioxidant and anti-inflammatory activities were reported in 91% of studies, while antimicrobial effects were documented in 69%.
Conclusion
The phytochemical richness of O. ficus-indica cladodes provides a strong scientific basis for their traditional and modern uses. Their pharmacological effects arise from synergistic interactions among multiple bioactive compounds acting through diverse mechanisms. Overall, cladodes represent a promising multifunctional resource for nutraceutical and therapeutic applications. However, further clinical studies are required to bridge the gap between preclinical findings and human applications.
References
Aalaoui, M. E., & Sbaghi, M. (2023). Morphological and phenological characterization of Moroccan Opuntia cactus varieties (Karama, Ghalia, Belara, Marjana, Cherratia, Angad, and Melk Zhar) resistant to the cactus cochineal Dactylopius opuntiae (Cockerell). Journal of the Professional Association for Cactus Development, 25, 115–135.
Abba, S., Muhammad, A., Ibrahim, A. A., Kurawa, M. S., Adamu, M. A., & Mu’azu, A. B. (2022). In vitro antioxidant and antibacterial properties of the leaf extracts of cactus plant (Opuntia ficus indica). International Journal of Research, Innovation and Applied Science, 7(7), 44–47. https://doi.org/10.51584/IJRIAS.2022.7703
Adjafre, B. L., Lima, I. C., Alves, A. P. N. N., Lessa, R. A., Cunha, A. P., Pereira, M. G., Mota, M. R., Santos, F. A., & Mota, M. R. L. (2024). Anti-inflammatory and healing effect of the polysaccharidic extract of O. ficus-indica cladodes in cutaneous excisional wounds in rats. International Journal of Experimental Pathology, 105(1), 33–44. https://doi.org/10.1111/iep.12498
Ahmed, F. A., Ibrahim, M. A., El-Azab, M. M., Fahmy, W. G. E., & Fahmy, D. M. (2024). A review: O. ficus-indica as a source of bioactive compound ingredients for functional foods, nutrition, human disease and health. Universal Journal of Pharmaceutical Research, 9(1), 52–61. https://doi.org/10.22270/ujpr.v9i1.1061
Alghamdi, A., Alshehri, W., Sajer, B., Ashkan, M., Ashy, R., Gashgari, R., & Hakmi, H. (2023). Biological activities and GC-MS analysis of Aloe vera and O. ficus-indica extracts. Journal of Chemistry, 2023, 1–15. https://doi.org/10.1155/2023/6504505
Alqudah, S. M., Hailat, M., Zakaraya, Z., Abu Dayah, A. A., Abu Assab, M., Alarman, S. M., ... Abu Dayyih, W. (2024). Impact of O. ficus-indica juice and empagliflozin on glycemic control in rats. Current Issues in Molecular Biology, 46(11), 12343–12353. https://doi.org/10.3390/cimb46110733
Ammam, A., Zemour, H., Kaid, M., Villemin, D., Soufan, W., & Belhouadjeb, F. A. (2023). Assessment of the anti-inflammatory and analgesic effects of Opuntia ficus indica L. cladodes extract. Libyan Journal of Medicine, 18(1), Article 2275417. https://doi.org/10.1080/19932820.2023.2275417
Amrane-Abider, M., Imre, M., Herman, V., Debbou-Iouknane, N., Saci, F., Boudries, H., ... Ayad, A. (2023). O. ficus-indica peel by-product as a natural antioxidant food additive and natural anticoccidial drug. Foods, 12(24), Article 4403. https://doi.org/10.3390/foods12244403
Aparicio-Ortuño, R., Jiménez-González, O., Lozada-Ramírez, J. D., & Ortega-Regules, A. E. (2024). Cladodes of Opuntia ficus indica as a functional ingredient in the production of cookies: Physical, antioxidant and sensory properties. Sustainable Food Technology, 2(4), 816–825. https://doi.org/10.1039/D4FB00019F
Arias Gorman, A. M., Monzon, C. M., La Cava, E. L., & Sgroppo, S. C. (2025). Interesting properties of Opuntia’s cladodes: An updated review. International Journal of Food Science and Technology, 60(1), Article vvaf046.
Aruwa, C. E., Amoo, S. O., & Kudanga, T. (2019). Extractable and macromolecular antioxidants of O. ficus-indica cladodes: Phytochemical profiling, antioxidant and antibacterial activities. South African Journal of Botany, 125, 402–410. https://doi.org/10.1016/j.sajb.2019.08.007
Benramdane, E., Mustafa, A., Chougui, N., Makhloufi, N., Tamendjari, A., & Mussagy, C. U. (2025). Identification, quantification, and antioxidant evaluation of phenolic compounds from colored O. ficus-indica (L.) roots using UHPLC-DAD-ESI-MS/MS. Antioxidants, 14(8), Article 1023. https://doi.org/10.3390/antiox14081023
Berhanu, B., & Khshen, Z. G. (2025). Experimental investigation of O. ficus-indica mucilage as a concrete admixture for sustainable construction. Ethiopian Journal of Science and Sustainable Development, 12(2), 14–22.
Brahmi, F., Oufighou, A., Smail-Benazzouz, L., Hammiche, N., Hassaine, L., Boulekbache-Makhlouf, L., ... Blando, F. (2024). Assessment of the chemical composition and antioxidant capacity of flowers, seeds, and seed cake of cactus pear (O. ficus-indica L.) and their application in biscuits. Resources, 13(9), Article 124. https://doi.org/10.3390/resources13090124
Caminiti, R., Serra, M., Nucera, S., Ruga, S., Oppedisano, F., Scarano, F., ... Maiuolo, J. (2024). Antioxidant activity and seasonal variations in the composition of insoluble fiber from the cladodes of O. ficus-indica (L.) Miller: Development of new extraction procedures to improve fiber yield. Plants, 13(4), Article 544. https://doi.org/10.3390/plants13040544
Elaloui, M., Ghazghazi, H., Ennajah, A., Smidi, H., Othman, N. B., & Laamouri, Ph. (2019). Phytoconstituents and biological activities of O. ficus-indica L. mucilage grown in three Tunisian provenances. Journal of Research in Environmental Science and Toxicology, 8(1), 16–24. https://doi.org/10.14303/jrest.2019.025
Elshewy, A., Blando, F., Bahlol, H., El-Desouky, A., De Bellis, P., & Khalifa, I. (2023). Egyptian O. ficus-indica (OFI) residues: Recovery and characterization of fresh mucilage from cladodes. Horticulturae, 9(6), Article 736. https://doi.org/10.3390/horticulturae9070736
Filannino, P., Cavoski, I., Thlien, N., Vincentini, O., De Angelis, M., Silano, M., ... Di Cagno, R. (2016). Lactic acid fermentation of cactus cladodes (O. ficus-indica L.) generates flavonoid derivatives with antioxidant and anti-inflammatory properties. PLOS ONE, 11(3), Article e0152575. https://doi.org/10.1371/journal.pone.0152575
Galati, E. M., Mondello, M. R., Monforte, M. T., Galluzzo, M., Miceli, N., & Tripodo, M. M. (2023). Effect of O. ficus-indica (L.) Mill. cladodes in the wound-healing process. Journal of the Professional Association for Cactus Development, 25, 78–92.
Heikal, A., Abd El-Sadek, M. E., Salama, A., & Taha, H. S. (2021). Comparative study between in vivo- and in vitro-derived extracts of cactus (O. ficus-indica L. Mill) against prostate and mammary cancer cell lines. Heliyon, 7(9), Article e08016. https://doi.org/10.1016/j.heliyon.2021.e08016
Hikal, W. M., Said-Al Ahl, H. A., & Kačániová, M. (2021). A review of antimicrobial activities of cactus. Asian Journal of Research in Biosciences, 3(2), 92–99.
Hwang, S. H., Kang, I.-J., & Lim, S. S. (2017). Antidiabetic effect of fresh nopal (O. ficus-indica) in low‐dose streptozotocin‐induced diabetic rats fed a high‐fat diet. Evidence-Based Complementary and Alternative Medicine, 2017, Article 4380721. https://doi.org/10.1155/2017/4380721
Iftikhar, K., Siddique, F., Ameer, K., Arshad, M., Kharal, S., Mohamed Ahmed, I. A., Yasmin, Z., & Aziz, N. (2023). Phytochemical profiling, antimicrobial, and antioxidant activities of hydroethanolic extracts of prickly pear (Opuntia ficus indica) fruit and pulp. Food Science & Nutrition, 11(4), 1916–1930. https://doi.org/10.1002/fsn3.3226
Jameel, S., Khan, M. A., Asif, A., Hameed, H., Ur Rahman, S., Irfan, A., Shazly, G. A., & Bin Jardan, Y. A. (2025). Exploring the antibacterial efficacy of Opuntia monacantha in combatting methicillin-resistant Staphylococcus aureus. Scientific Reports, 15, Article 9552. https://doi.org/10.1038/s41598-025-93939-w
Kabengele, C. N., Ngoyi, E. M., Kasiama, G. N., Kilembe, J. T., Matondo, A., Inkoto, C. L., ... Mpiana, P. T. (2020). Antihelminthic activity, phytochemical profile and microscopic features of Ocimum basilicum collected in DR Congo. Asian Journal of Biology, 10(3), 42–50. https://doi.org/10.9734/ajob/2020/v10i330110
Khémiri, I., Essghaier, H. B., Sadfi Zouaoui, N., Ben Gdara, N., & Bitri, L. (2019). The antimicrobial and wound healing potential of Opuntia ficus indica L. inermis extracted oil from Tunisia. Evidence-Based Complementary and Alternative Medicine, 2019, Article 9148782. https://doi.org/10.1155/2019/9148782
Lamia, S., Ammam, A., Kadda, H., & Reda, B. (2022). Polyphenol content, antioxidant and antibacterial activity of the aqueous extract of O. ficus-indica cladodes. Egyptian Academic Journal of Biological Sciences, C: Physiology & Molecular Biology, 14(2), 465–474. https://doi.org/10.21608/eajbsc.2022.245607
Lehner, M. D., Scheyhing, U., & Elsässer, J. (2024). A combination of carbonates and O. ficus-indica extract protects esophageal cells against simulated acidic and non-acidic reflux in vitro. Scientific Reports, 14, Article 22355. https://doi.org/10.1038/s41598-024-74047-7
Liu, X., Xing, Y., Liu, G., Bao, D., Hu, W., Bi, H., & Wang, M. (2025). Extraction, purification, structural features, biological activities, and applications of polysaccharides from O. ficus-indica (L.) Mill. (cactus): A review. Frontiers in Pharmacology, 16, Article 1566000. https://doi.org/10.3389/fphar.2025.1566000
López-Romero, P., Pichardo-Ontiveros, E., Avila-Nava, A., Vázquez-Manjarrez, N., Tovar, A. R., Pedraza-Chaverri, J., & Torres, N. (2014). The effect of nopal (Opuntia ficus indica) on postprandial blood glucose, incretins, and antioxidant activity in Mexican patients with type 2 diabetes after consumption of two different composition breakfasts. Journal of the Academy of Nutrition and Dietetics, 114(11), 1811–1818. https://doi.org/10.1016/j.jand.2014.06.352
Luna-Sosa, B., Martínez-Ávila, G. C. G., Rodríguez-Fuentes, H., Pastrana, L. M., Azevedo, A. G., González-Sandoval, D. C., ... Rojas, R. (2022). Extraction and characterization of mucilage from O. ficus-indica cultivated on hydroponic system. Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 50(1), Article 12460. https://doi.org/10.15835/nbha50112460
Maiuolo, J., Nucera, S., Serra, M., Caminiti, R., Oppedisano, F., Macrì, R., ... Mollace, V. (2024). Cladodes of O. ficus-indica (L.) Mill. possess important beneficial properties dependent on their different stages of maturity. Plants, 13(10), Article 1365. https://doi.org/10.3390/plants13101365
Martins, M., Ribeiro, M. H., & Almeida, C. M. M. (2023). Physicochemical, nutritional, and medicinal properties of O. ficus-indica (L.) Mill. and its main agro-industrial use: A review. Plants, 12(7), Article 1512. https://doi.org/10.3390/plants12071512
Mokrani, S., Ibrahim, N. A., Benaricha, B., Houali, K., Cruz, C., Boungab, K., ... Nabti, E. (2025). Phytochemical characterization and antifungal potential of O. ficus-indica cladode extracts against tomato pathogens. Processes, 13(5), Article 1412. https://doi.org/10.3390/pr13051412
Msaddak, L., Abdelhedi, O., Kridene, A., Rateb, M., Belbahri, L., Ammar, E., Nasri, M., & Zouari, N. (2017). O. ficus-indica cladodes as a functional ingredient: Bioactive compounds profile and their effect on antioxidant quality of bread. Lipids in Health and Disease, 16, Article 32. https://doi.org/10.1186/s12944-016-0397-y
Mylo, M. D., Hesse, L., Masselter, T., Leupold, J., Drozella, K., Speck, T., & Speck, O. (2021). Morphology and anatomy of branch–branch junctions in O. ficus-indica and Cylindropuntia bigelovii: A comparative study supported by mechanical tissue quantification. Plants, 10(11), Article 2313. https://doi.org/10.3390/plants10112313
Nefzaoui, A. (2019). O. ficus-indica productivity and ecosystem services in arid areas. Italus Hortus, 25(3), 29–39. https://doi.org/10.26353/j.itahort/2018.3.2939
Öncül, Ş., Becer, E., Mega Tiber, P., Teralı, K., & Aykac, A. (2024). In vitro and in silico investigations of the pro-apoptotic activity of O. ficus-indica cladode extracts against K562 cells. Turkish Journal of Biochemistry, 49(5), 533–541. https://doi.org/10.1515/tjb-2023-0229
Padilla-Camberos, E., Flores-Fernandez, J. M., Fernandez-Flores, O., Gutierrez-Mercado, Y., Carmona-de La Luz, J., Sandoval-Salas, F., ... Allen, K. (2015). Hypocholesterolemic effect and in vitro pancreatic lipase inhibitory activity of an O. ficus-indica extract. BioMed Research International, 2015, Article 837452. https://doi.org/10.1155/2015/837452
Pérez-Viveros, D. J., Díaz-Batalla, L., Navarro-Cortez, R. O., Palma-Rodríguez, H. M., & Hernández-Uribe, J. P. (2024). Effect of extrusion on physicochemical and functional properties of cladode flour from Opuntia cochenillifera and O. ficus-indica. Applied Food Research, 4(2), Article 100568. https://doi.org/10.1016/j.afres.2024.100568
Pulate, G. D., Damame, S. V., Wagh, J. G., Kale, A. A., & Lokhande, P. K. (2024). Assessment of nutritional value and in vitro anthelmintic activity in cactus (Opuntia ficus-indica (L.) Mill.) cladodes. International Journal of Advanced Biochemistry Research, 8(12), 1189–1195.
Quintero-García, M., Gutiérrez-Cortez, E., Bah, M., Rojas-Molina, A., Cornejo-Villegas, M. D. L. A., Del Real, A., & Rojas-Molina, I. (2021). Comparative analysis of the chemical composition and physicochemical properties of the mucilage extracted from fresh and dehydrated Opuntia ficus indica cladodes. Foods, 10(9), Article 2137. https://doi.org/10.3390/foods10092137
Rojas-Molina, I., Bah, M., Gutiérrez-Cortez, E., Rojas-Molina, A., Cornejo-Villegas, M. D. L. A., & Del Real, A. (2022). Functional properties of O. ficus-indica (L.) Mill. mucilage in food and pharmaceutical applications: A review. Foods, 11(9), Article 1234. https://doi.org/10.3390/foods11091234
Romero-Narvaez, A., González-Ríos, O., & Ruiz-López, I. I. (2019). Nutraceutical potential of cactus pear (O. ficus-indica) in human health. Nutrients, 11(10), Article 2453. https://doi.org/10.3390/nu11102453
Sadiq, M. B., Khattak, J. Z. K., Nisar, M., Rehman, H., Ali, R., & Akhtar, R. (2020). Antimicrobial activity of O. ficus-indica extracts against pathogenic bacteria and fungi. Journal of Applied Biology & Biotechnology, 8(1), 40–45. https://doi.org/10.7324/JABB.2020.8106
Sani, A. R., Muhammad, N., & Abubakar, S. (2021). Phytochemistry, nutritional value, and health benefits of prickly pear (O. ficus-indica). Food Science & Nutrition, 9(2), 1125–1135. https://doi.org/10.1002/fsn3.2351
Shabani, E., Karimi, E., & Asadollahi, M. (2024). Structural characterization and bioactive properties of polysaccharides extracted from O. ficus-indica cladodes. International Journal of Biological Macromolecules, 241, 124–137. https://doi.org/10.1016/j.ijbiomac.2023.12.012
Silva, A., Rosa, C., & Ferreira, A. (2022). Antioxidant and anti-inflammatory activities of O. ficus-indica fruit and cladode extracts. Food Chemistry, 378, Article 132063. https://doi.org/10.1016/j.foodchem.2021.132063
Sreedhar, R., & Sharma, R. (2023). Health-promoting compounds and functional properties of cactus (O. ficus-indica L.): A comprehensive review. Journal of Functional Foods, 105, Article 105488. https://doi.org/10.1016/j.jff.2023.105488
Teles, M., Ferreira, A., & Santos, J. (2021). Extraction and characterization of mucilage from O. ficus-indica for potential applications in food and pharma industries. Carbohydrate Polymers, 252, 117–126. https://doi.org/10.1016/j.carbpol.2020.117126
Valdez-Morales, M. L., Rojas-Molina, I., Bah, M., & Gutiérrez-Cortez, E. (2023). Technological applications of O. ficus-indica mucilage: A systematic review. Foods, 12(11), Article 2147. https://doi.org/10.3390/foods12092147
Vega-Castro, C., Hernández-Ramos, J., & Rodríguez-Félix, F. (2020). Chemical composition and antioxidant activity of O. ficus-indica fruit and cladodes grown in arid regions. Journal of Food Biochemistry, 44(7), Article e13240. https://doi.org/10.1111/jfbc.13240
Villegas-García, C., Aguilar, C., & Rivera, D. (2022). Opuntia species: Nutritional, medicinal, and industrial applications. Plant Foods for Human Nutrition, 77(3), 328–341. https://doi.org/10.1007/s11130-022-01006-9
Wang, X., Xu, J., Zhang, Y., & Chen, H. (2024). Advances in bioactive polysaccharides from O. ficus-indica: Structure, function, and potential health benefits. Carbohydrate Polymers, 319, 120–135. https://doi.org/10.1016/j.carbpol.2023.120135
Yahia, E. M., Hernández, D. A., & Pérez-López, J. A. (2021). Nutritional composition, bioactive compounds, and health benefits of O. ficus-indica (prickly pear). Food Research International, 143, Article 110260. https://doi.org/10.1016/j.foodres.2021.110260
Zambrano, J. L., Cruz, E., & Ramirez, A. (2022). Extraction methods and functional properties of O. ficus-indica mucilage for food and pharmaceutical use. Journal of Food Science, 87(2), 543–556. https://doi.org/10.1111/1750-3841.16135
Zouari, N., Abdelhedi, O., Msaddak, L., & Nasri, M. (2017). Chemical composition, bioactive compounds, and antioxidant activities of O. ficus-indica fruits and cladodes. Food Science & Nutrition, 5(6), 1022–1032.
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